Compound Isolation Systems
Sepbox 2D – 250
The Sepbox 2D-250 benchtop system is designed for the automated two-dimensional separation of up to 250 milligrams of extract. The system offers the choice between liquid and adsorbed (solid) sample injection. Importantly, up to 576 fractions can be collected in microtiter plates in just 24 hours. The Sepbox 2D-250 is suitable for medium to non-polar compounds (Polar Setup optional).
The system is equipped with intuitive, user-friendly software for method development, process control, data acquisition and data analysis.
The Sepbox 2D-250 offers high purity of fractions. On average, after one Sepbox run, up to 20 per cent of all fractions from plant extracts are 90 to 99 per cent pure (ELSD*), and from extracts made of marine organisms, up to 30 per cent are 90 to 99 per cent pure (ELSD).
As well as these highly purified compounds, it is usual to find a large number of fractions with compounds whose purity levels are between 50 and 90 per cent in ELSD detection. Results vary depending on the extract and the examined organism.
* The purity levels quoted here were calculated using the 100 per cent method with ELSD detection, as UV detection does not provide reliable purity levels due to different absorption coefficients.
• Isolation of compounds with a speed and accuracy unachievable with conventional chromatography methods
• On average 20 to 30 per cent of fractions achieve 90 to 99 per cent purity (ELSD) per run
• Isolation of large quantities possible
• Reproducible separations
• Cost advantages due to automation
With the Sepbox 2D-2000 benchtop system up to 2,000 milligrams of extract can be processed and is equipped with two detectors: UV detection (ultraviolet) and optional ELSD detection (evaporative light scattering detection), which can detect almost any substance. If required, highly polar substances can be separated using the Polar Setup.
Due to the automated and highly reproducible process, one extract can be completely separated per day using two-dimensional separation. The recovery rate for both polar and non-polar substances is usually above 90 per cent.
Up to 576 fractions with a very high yield of pure compounds can be collected in vials for subsequent High Throughput Screening.
Today, HPLC is the standard technology used for separating compounds from natural resources. The Sepbox concept is based on a patented combination of HPLC and SPE (Solid Phase Extraction) and combines proven techniques with reliability to provide a universal platform suitable for processing large sample numbers.
Sepiatec’s unique Sepbox systems allow samples to be processed automatically and significantly accelerate the purification process. Results that can usually take weeks or months to achieve via conventional methods can be obtained in just a few days – or even one day – with a Sepbox system. Up to 576 fractions with a very high yield of pure compounds can be efficiently obtained for subsequent High Throughput Screening. Using two-dimensional separation, the recovery rate for both polar and non-polar substances is usually above 90 per cent.
Using an automated and highly reproducible process, one extract can be completely separated per day. The pure individual compounds are soluted in suitable solvents and can be collected in vials (Sepbox 2D-5000 and Sepbox 2D-2000) or microtiter plates (Sepbox 2D-250).
The Sepbox 2D-5000 is designed for a large workload of up to 5,000 milligrams of extract made from plants, marine organisms, microbiological or animal material. The automated 2D chromatography provides 576 fractions in 24 hours. The fraction collector is equipped with 12 removable racks, each containing 48 vials with a maximum capacity of 60 millilitres.
The Sepbox 2D-5000 is equipped with software (controlled from a touchscreen on the front of the machine) for method development, process control, data acquisition and data analysis.
The Sepbox 2D-5000 provides many individual chemical entities in quantities which can be easily used to elucidate structures, screen different targets and build a diverse library.
For more information or to request a quote: